Miscellaneous  

Current projects
 
Requests regarding scientific cooperations or MD and PhD theses, respectively, are welcome at any time! For the latter you can usually join us immediately for collaboration in several different exciting projects.
 
  • Molecular Imaging
   Within the scope of this project various "cell tracking" experiments should be carried out. Aim of this study is an "online monitoring" of labelled cells. For this two different approaches can be considered: On the one hand cells can be loaded with perfluorocarbons (PFCs), in order to detect the cells subsequently with combined 1H/19F MRI in the body as "Hot Spot". On the other hand cells can be loaded with superparamagnetic iron oxide particles (SPIOs). Owing to their small size (about 30 nm) these particles fluctuate in the magnetic field and as a consequence of the permanently changing magnetization direction, they increase the local inhomogeneity inside the field. This results in a localized enhanced T2* effect leading to a hypointense region in the MR image, so that crabwise can be deduced on the presence of the labelled cells.
  • Angiography
   Aim of this project is to further improve the image quality for angiographic studies and to volumetrically quantify alterations in the vessel structure. The optimized protocols should be applied in an experimental series about neovascularization processes in CD73-/- mice, in order to elucidate the role of extracellularly formed adenosine in arterio. and angiogenesis, respectively.
  • Plaque imaging
   While in the above-mentioned project luminal changes in the vascular system should be visualized, this study will focus on the vessel wall. For this purpose "black blood" sequences will be implemented to generate contrast between wall and inner of the vessel, so that alterations in the architecture of the vascular wall can be observed over time. Furthermore, it should be investigated, whether the parallel administration of PFCs will allow the visualization of inflammatory processes within the plaque by combined 1H/19F MRI. The developed approaches will then be used to investigate the temporal development of arteriosclerosis in CD73-/- mice and CD73-/-/ApoE-/- double mutants, respectively.
  • Spectroscopic 19F imaging
   All previous experiments for visualization of inflammatory processes via 1H/19F MRI were carried out with the PFC perfluoro-15-crown-5 ether, a small, cyclic molecule, in which all 20 fluorine nuclei are chemical equivalent. The advantage is smooth 19F MR imaging, but its extremely long biological retention time does not allow the use of this compound for repetitive investigations and clinical studies, respectively. Therefore, other PFCs should be investigated, which are known to be nontoxic, to be readily exhaled via the lung, and to have a biological half-life of only a few days (e.g. perfluorooctyl bromide, perfluorodecalin). However, the presence of different fluorine nuclei within these molecules necessitate the implementation of fast spectroscopic imaging methods for sensitive and artefact-free imaging of these PFCs.
 
 
 
Funding
  		 
  • DFG-Einzelantrag
   Simultaneous detection of multiple structures by 19F MRI for in vivo assessment of thrombus stage and inflammatory thrombosis – TE1209/1-1 + FL303/6-1
  Master switches in cardiac ischemia
Project B02: Pro- und anti-inflammatory effects of interleukins in cardiac ischemia:
Project B05: Interrelationships between fatty liver disease/insulin resistance and tolerance to cardiac ischemia
  • DFG* research grant
   Development of perfluorcarbon nanoemulsions for selective targeting and visualization of specific epitopes and cell types by in vivo 19F-MR – SCHR154/13-2
  Molecular analysis of cardiovascular functions and dysfunctions
Project Z2: Cardiovascular phenotyping of transgenic mice
  • Graduiertenkolleg* 1089
   Protein interactios and modifications in the heart
Project 1: Interactions of myoglobin with cytosolic proteins and its role in cardiac substrate selection
  • DFG research grant
   Development of nanoemulsions from per- and semifluorinated alkanes for in vivo 19F MR diagnostik of inflammatory processes – SCHR154/13-1
  • DFG research grant
   Local cardiac function and metabolism of transgenic mice by high resolution magnetic resonance techniques – SCHR154/9-2 and SCHR154/9-3
  • DFG research grant
   Assessment of heart function and ventricle geometry in transgenic mice by high resolution magnetic resonanve imaging (MRI) – SCHR154/9-1
  • NIH research grant
   Role of inflammation in pulmonary injury – National Heart, Lung and Blood Institute, UVA Charlottesville, VIII 04- VII 09
  • Research commission
    HHU* Düsseldorf
   Development of cardiomyopathy caused by deficiency of very long chain acyl CoA dehydrogenase (VLCAD)
  • Research commission
    HHU Düsseldorf
   Evaluation of new experimental approaches for gene and cell therapy of the heart
  • *Abbreviations and translations: Sonderforschungsbereich, Collaborative Research Center; Graduiertenkolleg, Research Training Group; Deutsche Forschungsgemeinschaft (DFG), German Research Foundation; HHU, Heinrich Heine University
 
 
Co-operation partner
  		 
  • Prof. Volker Aurich
   Institute for Informatics, Heinrich Heine University Düsseldorf – Quantitative volumetric analysis of 3D MR angiographic data sets
  • Prof. Stefan Egelhaaf
   Physics of soft matter, Heinrich Heine University Düsseldorf – Phenomenons at the interface between detergents and water
  • Prof. Jens Fischer
   Institute for Pharmacology and Clinical Pharmacology, University Hospital Düsseldorf – Consequences of biglycan deficiency during myocardial ischemia
  • Prof. Norbert Frey
   Internal Medicine 3, University Hospital Kiel – Role of cardiac Troponin T in hypertrophic cardiomyopathy
  • Prof. Dieter Häussinger
   Clinic for Gastroenterology, Hepatology, and Infectiology, University Hospital Düsseldorf – Effect of taurine paucity in skeletal and heart muscle
  • Prof. Thomas Hohlfeld
   Institute for Pharmacology and Clinical Pharmacology, University Hospital Düsseldorf – Cardiovascular Phenotyping of EP3 receptor-overexpressing mice
  • Prof. Sebastian Jander
   Neurological Clinic, University Hospital Düsseldorf – Investigation of stroke models
  • Prof. Michaela Kuhn
   Department of Physiology, University Würzburg – Effects of natriuretic peptides on angiogenesis
  • Prof. Florian Lang
   Institute for Physiology, Eberhard Karls University Tübingen – Development of hypertophy in a serine-threonine-kinase-1 (SGK1) deficient mouse modell
  • Prof. Bodo Levkau
   Institute for Pathophysiology, University Duisburg-Essen – Heart function of survivin knockout mice
  • Prof. Joel Linden
   Department of Medicine, University of Virginia, Charlottesville – Role of inflammatory processes in pulmonal diseases
  • Prof. Gabriele Pfitzer
   Vegetative Physiology, University Cologne – Functional characterisation of cardiomyopathy-relevant mutations and troponin I
  • Prof. Ulrich Rüther
   Institute for Development and Molecular Biology of Animals, Heinrich Heine University Düsseldorf – Role of Irx genes in heart development
  • Prof. Michael Schäfers
   Nuclear Medicine, University Hospital Münster – Correlation of MR and PET investigations on transgenic mice
  • Prof. Rolf Schubert
   Pharmaceutical Technology and Biopharmacy, Albert Ludwigs University Freiburg – Preparation and characterization of perfluorocarbon emulsions for 19F MR imaging
  • Prof. Ute Spiekerkötter
   Clinic for Pediatrics, University Hospital Freiburg – Development of cardiomyopathy caused by deficiency of very long chain acyl CoA dehydrogenase
 
 
Publications
  		 
 
The citations are listed in chronological order with the latest papers at the top. For articles related to special issues, see the individual areas for heart, vessels, fluorine, energetics, metabolism and myoglobin, respectively. The references are linked with the PubMed abstracts of the National Library of Medicine. If you are interested in one of these paper, and you do not have online access to the particular juornal, send us an email and we will provide you with the pdf-file.
 
Flögel U, Schlüter A, Jacoby C, Temme S, Banga JP, Eckstein A, Schrader J, Berchner-Pfannschmidt U.
Multimodal assessment of orbital immune cell infiltration and tissue remodeling during development of graves disease by 1H/19F MRI.
Magn Reson Med. 2018; 80: 711-718.
 
Güden-Silber T, Temme S, Jacoby C, Flögel U.
Biomedical 19F MRI using perfluorocarbons.
Methods Mol Biol. 2018; 1718: 235-257.
 
Weske S, Vaidya M, Reese A, von Wnuck Lipinski K, Keul P, Bayer JK, Fischer JW, Flögel U, Nelsen J, Epple M, Scatena M, Schwedhelm E, Dörr M, Völzke H, Moritz E, Hannemann A, Rauch BH, Gräler MH, Heusch G, Levkau B.
Targeting sphingosine-1-phosphate lyase as an anabolic therapy for bone loss.
Nat Med. 2018; 24: 667-678.
 
Heinen A, Raupach A, Behmenburg F, Hölscher N, Flögel U, Kelm M, Kaisers W, Nederlof R, Huhn R, Gödecke A.
Echocardiographic analysis of cardiac function after infarction in mice: Validation of single-plane long-axis view measurements and the bi-plane Simpson method.
Ultrasound Med Biol. 2018; 44: 1544-1555.
 
Schlüter A, Horstmann M, Diaz-Cano S, Plöhn S, Stähr K, Mattheis S, Oeverhaus M, Lang S, Flögel U, Berchner-Pfannschmidt U, Eckstein A, Banga JP.
Genetic immunization with mouse thyrotrophin hormone receptor plasmid breaks self-tolerance for a murine model of autoimmune thyroid disease and Graves' orbitopathy.
Clin Exp Immunol. 2018; 191: 255-267.
 
Erkens R, Suvorava T, Sutton TR, Fernandez BO, Mikus-Lelinska M, Barbarino F, Flögel U, Kelm M, Feelisch M, Cortese-Krott MM.
Nrf2 deficiency unmasks the significance of nitric oxide synthase activity for cardioprotection.
Oxid Med Cell Longev. 2018; 2018:8309698 (15 pages).
 
Haberkorn SM, Jacoby C, Ding Z, Keul P, Bönner F, Polzin A, Levkau B, Schrader J, Kelm M, Flögel U.
Cardiovascular magnetic resonance relaxometry predicts regional functional outcome after experimental myocardial infarction.
Circ Cardiovasc Imaging. 2017; 10: pii e006025 (12 pages).
 
Borg N, Alter C, Görldt N, Jacoby C, Ding Z, Steckel B, Quast C, Bönner F, Friebe D, Temme S, Flögel U, Schrader J.
CD73 on T-cells orchestrates cardiac wound healing after myocardial infarction by purinergic metabolic reprogramming.
Circulation. 2017; 136: 297-313.
 
Jelenik T, Kaul K, Séquaris G, Flögel U, Phielix E, Kotzka J, Knebel B, Fahlbusch P, Hörbelt T, Lehr S, Reinbeck AL, Müller-Wieland D, Esposito I, Shulman GI, Szendroedi J, Roden M.
Mechanisms of insulin resistance in primary and secondary non-alcoholic fatty liver.
Diabetes. 2017; 66: 2241-2253.
 
Behmenburg F, Hölscher N, Flögel U, Hollmann MW, Heinen A, Huhn R.
Opening of calcium-activated potassium channels improves long-term left-ventricular function after coronary artery occlusion in mice.
Int J Cardiol. 2017; 241: 351-357.
 
Tucci S, Flögel U, Beermann F, Behringer S, Spiekerkoetter U.
Triheptanoin: long-term effects in the very long-chain acyl-CoA dehydrogenase-deficient mouse.
J Lipid Res. 2017; 58: 196-207.
 
Hendgen-Cotta UB, Esfeld S, Coman C, Ahrends R, Klein-Hitpass L, Flögel U, Rassaf T, Totzeck M.
A novel physiological role for cardiac myoglobin in lipid metabolism.
Sci Rep. 2017; 7: 43219 (13 pages).
 
Haddad S, Wang Y, Galy B, Korf-Klingebiel M, Hirsch V, Baru AM, Rostami F, Reboll MR, Heineke J, Flögel U, Groos S, Renner A, Toischer K, Zimmermann F, Engeli S, Jordan J, Bauersachs J, Hentze MW, Wollert KC, Kempf T.
Iron-regulatory proteins secure iron availability in cardiomyocytes to prevent heart failure.
Eur Heart J. 2017; 38: 362-372.
 
Colotti R, Bastiaansen JA, Wilson A, Flögel U, Gonzales C, Schwitter J, Stuber M, van Heeswijk RB.
Characterization of perfluorocarbon relaxation times and their influence on the optimization of fluorine-19 MRI at 3 Tesla.
Magn Reson Med. 2017; 77: 2263-2271.
 
Rammos C, Hendgen-Cotta UB, Totzeck M, Pohl J, Lüdike P, Flögel U, Deenen R, Köhrer K, French BA, Gödecke A, Kelm M, Rassaf T.
Impact of dietary nitrate on age-related diastolic dysfunction.
Eur J Heart Fail. 2016; 18: 599-610.
 
Bönner F, Spieker M, Haberkorn S, Jacoby C, Flögel U, Schnackenburg B, Horn P, Reinecke P, Neizel-Wittke M, Kelm M, Westenfeld R.
Myocardial T2 mapping increases noninvasive diagnostic accuracy for biopsy-proven myocarditis.
JACC Cardiovasc Imaging. 2016; 9: 1467-1469
 
Ding Z, Temme S, Quast C, Friebe D, Jacoby C, Zanger K, Bidmon HJ, Grapentin C, Schubert R, Flögel U, Schrader J.
Epicardium-derived cells formed after myocardial injury display phagocytic activity permitting in vivo labeling and tracking.
Stem Cells Transl Med. 2016; 5: 639-650.
 
Temme S, Grapentin C, Quast C, Jacoby C, Grandoch M, Ding Z, Owenier C, Mayenfels F, Fischer JW, Schubert R, Schrader J, Flögel U.
Noninvasive imaging of early venous thrombosis by 19F magnetic resonance imaging with targeted perfluorocarbon nanoemulsions.
Circulation. 2015; 131: 1405-14.
 
van Heeswijk RB, Pellegrin M, Flögel U, Gonzales C, Aubert JF, Mazzolai L, Schwitter J, Stuber M.
Fluorine MR imaging of inflammation in atherosclerotic plaque in vivo.
Radiology. 2015; 275: 421-9.
 
Tucci S, Flögel U, Spiekerkoetter U.
Sexual dimorphism of lipid metabolism in very long-chain acyl-CoA dehydrogenase deficient (VLCAD(-/-)) mice in response to medium-chain triglycerides (MCT).
Biochim Biophys Acta. 2015; 1852: 1442-50.
 
Bönner F, Merx MW, Klingel K, Begovatz P, Flögel U, Sager M, Temme S, Jacoby C, Salehi Ravesh M, Grapentin C, Schubert R, Bunke J, Roden M, Kelm M, Schrader J.
Monocyte imaging after myocardial infarction with 19F MRI at 3 T: a pilot study in explanted porcine hearts.
Eur Heart J Cardiovasc Imaging. 2015; 16: 612-20.
 
Bönner F, Janzarik N, Jacoby C, Spieker M, Schnackenburg B, Range F, Butzbach B, Haberkorn S, Westenfeld R, Neizel-Wittke M, Flögel U, Kelm M.
Myocardial T2 mapping reveals age- and sex-related differences in volunteers.
J Cardiovasc Magn Reson. 2015; 17: 9.
 
Burghoff S, Gong X, Viethen C, Jacoby C, Flögel U, Bongardt S, Schorr A, Hippe A, Homey B, Schrader J.
Growth and metastasis of B16-F10 melanoma cells is not critically dependent on host CD73 expression in mice.
BMC Cancer. 2014; 14: 898.
 
Bönner F, Jacoby C, Temme S, Borg N, Ding Z, Schrader J, Flögel U.
Multifunctional MR monitoring of the healing process after myocardial infarction.
Basic Res Cardiol. 2014; 109: 430.
 
Temme S, Jacoby C, Ding Z, Bönner F, Borg N, Schrader J, Flögel U.
Monitoring the trafficking of neutrophil granulocytes and monocytes during the course of tissue inflammation by noninvasive 19F MRI.
J Leukoc Biol. 2014; 95: 689-97.
 
Jacoby C, Temme S, Mayenfels F, Benoit N, Krafft MP, Schubert R, Schrader J, Flögel U.
Probing different perfluorocarbons for in vivo inflammation imaging by 19F MRI: image reconstruction, biological half-lives and sensitivity.
NMR Biomed. 2014; 27: 261-71.
 
Jacoby C, Borg N, Heusch P, Sauter M, Bönner F, Kandolf R, Klingel K, Schrader J, Flögel U.
Visualization of immune cell infiltration in experimental viral myocarditis by 19F MRI in vivo.
Magn Reson Mater Phy. 2014; 27: 101-6.
 
Tucci S, Flögel U, Hermann S, Sturm M, Schäfers M, Spiekerkoetter U.
Development and pathomechanisms of cardiomyopathy in very long-chain acyl-CoA dehydrogenase deficient VLCAD(-/-) mice.
Biochim Biophys Acta. 2014; 1842: 677-85.
 
Luedde M, Lutz M, Carter N, Sosna J, Jacoby C, Vucur M, Gautheron J, Roderburg C, Borg N, Reisinger F, Hippe HJ, Linkermann A, Wolf MJ, Rose-John S, Lüllmann-Rauch R, Adam D, Flögel U, Heikenwaelder M, Luedde T, Frey N.
RIP3, a kinase promoting necroptotic cell death, mediates adverse remodeling after myocardial infarction.
Cardiovasc Res. 2014; 103: 206-16.
 
Kukat A, Dogan SA, Edgar D, Mourier A, Jacoby C, Maiti P, Mauer J, Becker C, Senft K, Wibom R, Kudin AP, Hultenby K, Flögel U, Rosenkranz S, Ricquier D, Kunz WS, Trifunovic A.
Loss of UCP2 attenuates mitochondrial dysfunction without altering ROS rroduction and uncoupling activity.
PLoS Genet. 2014; 10: e1004385.
 
Böring YC, Flögel U, Jacoby C, Heil M, Schaper W, Schrader J.
Lack of ecto-5'-nucleotidase (CD73) promotes arteriogenesis.
Cardiovasc Res. 2013; 97: 88-96.
 
Burghoff S, Flögel U, Bongardt S, Burkart V, Sell H, Tucci S, Ikels K, Eberhard D, Kern M, Klöting N, Eckel J.
Deletion of CD73 promotes dyslipidemia and intramyocellular lipid accumulation in muscle of mice.
Arch Physiol Biochem. 2013; 119: 39-51.
 
Bönner F, Borg N, Jacoby C, Temme S, Ding Z, Flögel U, Schrader J.
Ecto-5'-nucleotidase on immune cells protects from adverse cardiac remodeling.
Circ Res. 2013; 113: 301-12.
 
Flögel U, Burghoff S, van Lent PL, Temme S, Galbarz L, Ding Z, El-Tayeb A, Huels S, Bönner F, Borg N, Jacoby C, Müller CE, van den Berg WB, Schrader J.
Selective activation of adenosine A2A receptors on immune cells by a CD73-dependent prodrug suppresses joint inflammation in experimental rheumatoid arthritis.
Sci Transl Med. 2012; 4: 146ra108.
 
Temme S, Bönner F, Schrader J, Flögel U.
19F magnetic resonance imaging of endogenous macrophages in inflammation.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2012; 4: 329-43.
 
Assmann A, Akhyari P, Delfs C, Flögel U, Jacoby C, Kamiya H, Lichtenberg A.
Development of a growing rat model for the in vivo assessment of engineered aortic conduits.
J Surg Res. 2012; 176: 367-75.
 
Tucci S, Flögel U, Sturm M, Borsch E, Spiekerkoetter U.
Disrupted fat distribution and composition due to medium-chain triglycerides in mice with a β-oxidation defect
Am J Clin Nutr. 2011; 94: 439-49.
 
Jacoby C, Flögel U.
MR for the investigation of murine vasculature.
Methods Mol Biol. 2011; 771: 439-56.
 
Borst O, Ochmann C, Schönberger T, Jacoby C, Stellos K, Seizer P, Flögel U, Lang F, Gawaz M.
Methods employed for induction and analysis of experimental myocardial infarction in mice.
Cell Physiol Biochem. 2011; 28: 1-12.
 
Flögel U, Su S, Kreideweiß I, Ding Z, Galbarz L, Fu J, Jacoby C, Witzke O, Schrader J.
Noninvasive detection of graft rejection by in vivo 19F MRI in the early stage.
Am J Transplant. 2011; 11: 235-44.
 
Mersmann J, Habeck K, Latsch K, Zimmermann R, Jacoby C, Fischer JW, Hartmann C, Schrader J, Kirschning CJ, Zacharowski K.
Left ventricular dilation in toll-like receptor 2 deficient mice after myocardial ischemia/reperfusion through defective scar formation.
Basic Res Cardiol. 2011; 106: 89-98.
 
Ebner B, Behm P, Jacoby C, Burghoff S, French BA, Schrader J, Flögel U.
Early assessment of pulmonary inflammation by 19F MRI in vivo.
Circ Cardiovasc Imaging. 2010; 3: 202-10.
 
Waclawczyk S, Buchheiser A, Flögel U, Radke TF, Kögler G.
In vitro differentiation of unrestricted somatic stem cells into functional hepatic-like cells displaying a hepatocyte-like glucose metabolism.
J Cell Physiol. 2010; 225: 545-54.
 
Pissarek M, Meyer-Kirchrath J, Hohlfeld T, Vollmar S, Oros-Peusquens AM, Flögel U, Jacoby C, Krügel U, Schramm N.
Targeting murine heart and brain: visualisation conditions for multi-pinhole SPECT with 99mTc- and 123I-labelled probes.
Eur J Nucl Med Mol Imaging. 2009; 36: 1495-509.
 
Kuhn M, Völker K, Schwarz K, Carbajo-Lozoya J, Flögel U, Jacoby C, Stypmann J, van Eickels M, Gambaryan S, Hartmann M, Werner M, Wieland T, Schrader J, Baba HA.
The natriuretic peptide/guanylyl cyclase - a system functions as a stress-responsive regulator of angiogenesis in mice.
J Clin Invest. 2009; 119: 2019-30.
 
Luedde M, Flögel U, Knorr M, Grundt C, Hippe HJ, Brors B, Frank D, Haselmann U, Antony C, Voelkers M, Schrader J, Most P, Lemmer B, Katus HA, Frey N.
Decreased contractility due to energy deprivation in a transgenic rat model of hypertrophic cardiomyopathy.
J Mol Med. 2009; 87: 411-22.
 
Meyer-Kirchrath J, Martin M, Schooss C, Jacoby C, Flögel U, Marzoll A, Fischer J, Schrader J, Schrör K, Hohlfeld T
Overexpression of prostaglandin EP3 receptors activates calcineurin and promotes hypertrophy in the murine heart.
Cardiovasc Res. 2009; 81: 310-8.

 

Jacoby C, Böring YC, Beck A, Zernecke A, Aurich V, Weber C, Schrader J, Flögel U.
Dynamic changes in murine vessel geometry assessed by high-resolution magnetic resonance angiography: a 9.4T study.
J Magn Reson Imaging. 2008; 28: 637-45.
 
Flögel U, Ding Z, Hardung H, Jander S, Reichmann G, Jacoby C, Schubert R, Schrader J.
In vivo monitoring of inflammation after cardiac and cerebral ischemia by fluorine magnetic resonance imaging.
Circulation. 2008; 118: 140-48.
 
Levkau B, Schäfers M, Wohlschlaeger J, von Wnuck Lipinski K, Keul P, Hermann S, Kawaguchi N, Kirchhof P, Fabritz L, Stypmann J, Stegger L, Flögel U, Schrader J, Fischer J, Hsieh P, Ou YL, Mehrhof F, Tiemann K, Ghanem A, Matus M, Neumann J, Heusch G, Schmid KW, Conway EM, Baba HA.
Survivin determines cardiac function by controlling total cardiomyocyte number.
Circulation. 2008; 117: 1583-93.
 
Westermann D, Mersmann J, Melchior A, Freudenberger T, Petrik C, Schaefer L, Lullmann-Rauch R, Lettau O, Jacoby C, Schrader J, Brand-Herrmann SM, Young MF, Schultheiss HP, Levkau B, Baba HA, Unger T, Zacharowski K, Tschope C, Fischer JW.
Biglycan is required for adaptive remodeling after myocardial infarction.
Circulation. 2008; 117: 1269-76.
 
Flögel U, Jacoby C, Gödecke A, Schrader J.
In vivo 2D mapping of impaired murine cardiac energetics in NO-induced heart failure.
Magn Reson Med. 2007; 57: 50-8.
 
Rassaf T, Flögel U, Drexhage C, Hendgen-Cotta U, Kelm M, Schrader J.
Nitrite reductase function of deoxymyoglobin: oxygen sensor and regulator of cardiac energetics and function.
Circ Res. 2007; 100: 1749-54.
 
Rockel N, Esser C, Grether-Beck S, Warskulat U, Flögel U, Schwarz A, Schwarz T, Yarosh D, Häussinger D, Krutmann J.
The osmolyte taurine protects against ultraviolet B radiation-induced immunosuppression.
J Immunol. 2007; 179: 3604-12.
 
Jacoby C, Molojavyi A, Flögel U, Merx MW, Ding Z, Schrader J.
Direct comparison of magnetic resonance imaging and conductance microcatheter in the evaluation of left ventricular function in mice.
Basic Res Cardiol. 2006; 101: 87-95.
 
Warskulat U, Borsch E, Reinehr R, Heller-Stilb B, Monnighoff I, Buchczyk D, Donner M, Flögel U, Kappert G, Soboll S, Beer S, Pfeffer K, Marschall HU, Gabrielsen M, Amiry-Moghaddam M, Ottersen OP, Dienes HP, Häussinger D.
Chronic liver disease is triggered by taurine transporter knockout in the mouse.
FASEB J. 2006; 20: 574-6.
 
Vallon V, Wyatt AW, Klingel K, Huang DY, Hussain A, Berchtold S, Friedrich B, Grahammer F, Belaiba RS, Görlach A, Wulff P, Daut J, Dalton ND, Ross J Jr, Flögel U, Schrader J, Osswald H, Kandolf R, Kuhl D, Lang F.
SGK1-dependent cardiac CTGF formation and fibrosis following DOCA treatment.
J Mol Med. 2006; 84: 396-404.
 
Flögel U, Laussmann T, Gödecke A, Abanador N, Schäfers M, Fingas CD, Metzger S, Levkau B, Jacoby C, Schrader J.
Lack of myoglobin causes a switch in cardiac substrate selection.
Circ Res. 2005; 96: e68-75.
 
Stegger L, Schäfers KP, Flögel U, Livieratos L, Hermann S, Jacoby C, Keul P, Conway EM, Schober O, Schrader J, Levkau B, Schäfers M.
Monitoring left ventricular dilation in mice with PET.
J Nucl Med. 2005; 46: 1516-21.
 
Martin M, Meyer-Kirchrath J, Kaber G, Jacoby C, Flögel U, Schrader J, Rüther U, Schrör K, Hohlfeld T.
Cardiospecific overexpression of the prostaglandin EP3 receptor attenuates ischemia-induced myocardial injury.
Circulation. 2005; 112: 400-6.
 
Merx MW, Gödecke A, Flögel U, Schrader J.
Oxygen supply and nitric oxide scavenging by myoglobin contribute to exercise endurance and cardiac function.
FASEB J. 2005; 19: 1015-7.
 
Limbourg FP, Ringes-Lichtenberg S, Schaefer A, Jacoby C, Mehraein Y, Jager MD, Limbourg A, Fuchs M, Klein G, Ballmaier M, Schlitt HJ, Schrader J, Hilfiker-Kleiner D, Drexler H.
Haematopoietic stem cells improve cardiac function after infarction without permanent cardiac engraftment.
Eur J Heart Fail. 2005; 7: 722-9.
 
Koszalka P, Özüyaman B, Huo Y, Zernecke A, Flögel U, Braun N, Buchheiser A, Decking UK, Smith ML, Sévigny J, Gear A, Weber AA, Molojavyi A, Ding Z, Weber C, Ley K, Zimmermann H, Gödecke A, Schrader J.
Targeted disruption of CD73/ecto-5'-nucleotidase alters thromboregulation and augments vascular inflammatory response.
Circ Res. 2004; 95: 814-21.
 
Flögel U, Gödecke A, Klotz LO, Schrader J.
Role of myoglobin in the antioxidant defense of the heart.
FASEB J. 2004; 18: 1156-8.
 
Jacoby C, Molojavyi A, Schrader J, Flögel U, Meyer-Kirchrath J, Hohlfeld T.
Cardiac MRI of transgenic mice.
Bruker Spin Report. 2004; 154/155: 49-53.
 
Warskulat U, Flögel U, Jacoby C, Hartwig HG, Thewissen M, Merx MW, Molojavyi A, Heller-Stilb B, Schrader J, Häussinger D.
Taurine transporter knockout depletes muscle taurine levels and results in severe skeletal muscle impairment but leaves cardiac function uncompromised.
FASEB J. 2004; 18: 577-9.
 
Schlieper G, Kim JH, Molojavyi A, Jacoby C, Laussmann T, Flögel U, Gödecke A, Schrader J.
Adaptation of the myoglobin knockout mouse to hypoxic stress.
Am J Physiol Regul Integr Comp Physiol. 2004; 286: R786-92.
 
Wunderlich C, Flögel U, Gödecke A, Heger J, Schrader J.
Acute inhibition of myoglobin impairs contractility and energy state of iNOS-overexpressing hearts.
Circ Res. 2003; 92: 1352-8.
 
Gödecke A, Molojavyi A, Heger J, Flögel U, Ding Z, Jacoby C, Schrader J.
Myoglobin protects the heart from inducible nitric-oxide synthase iNOS-mediated nitrosative stress.
J Biol Chem. 2003; 278: 21761-6.
 
Kojic ZZ, Flögel U, Schrader J, Decking UK.
Endothelial NO formation does not control myocardial O2 consumption in mouse heart.
Am J Physiol Heart Circ Physiol. 2003; 285: H392-7.
 
Heger J, Gödecke A, Flögel U, Merx MW, Molojavyi A, Kühn-Velten WN, Schrader J.
Cardiac-specific overexpression of inducible nitric oxide synthase does not result in severe cardiac dysfunction.
Circ Res. 2002; 90: 93-9.
 
Merx MW, Flögel U, Stumpe T, Gödecke A, Decking UK, Schrader J.
Myoglobin facilitates oxygen diffusion.
FASEB J. 2001;15: 1077-9.
 
Flögel U, Merx MW, Gödecke A, Decking UK, Schrader J.
Myoglobin: A scavenger of bioactive NO.
Proc Natl Acad Sci USA. 2001; 98: 735-40.
 
Zwingmann C, Flögel U, Pfeuffer J, Leibfritz D.
Effects of ammonia exposition on glioma cells: changes in cell volume and organic osmolytes studied by diffusion-weighted and high-resolution NMR spectroscopy.
Dev Neurosci. 2000; 22: 463-71.
 
Leibfritz D, Pfeuffer J, Flögel U, Meier C, Bröer S.
No Cell volume and water exchange in neural cells monitored by diffusion weighted 1H NMR spectroscopy.
Prog Brain Res. 2000; 125: 255-63.
 
Gödecke A, Flögel U, Zanger K, Ding Z, Hirchenhain J, Decking UK, Schrader J.
Disruption of myoglobin in mice induces multiple compensatory mechanisms.
Proc Natl Acad Sci USA. 1999; 96: 10495-500.
 
Flögel U, Decking UK, Gödecke A, Schrader J.
Contribution of NO to ischemia-reperfusion injury in the saline-perfused heart: a study in endothelial NO synthase knockout mice.
J Mol Cell Cardiol. 1999; 31: 827-36.
 
Pfeuffer J, Bröer S, Bröer A, Lechte M, Flögel U, Leibfritz D.
Expression of aquaporins in Xenopus laevis oocytes and glial cells as detected by diffusion-weighted 1H NMR spectroscopy and photometric swelling assay.
Biochim Biophys Acta. 1998; 1448: 27-36.
 
Flögel U, Leibfritz D.
Alterations in glial cell metabolism during recovery from chronic osmotic stress.
Neurochem Res. 1998; 23: 1553-61.
 
Brand A, Richter-Landsberg C, Flögel U, Willker W, Leibfritz D.
Rat brain primary neurons immobilized in basement membrane gel threads: an improved method for on-line 13C NMR spectroscopy of live cells.
Brain Res Brain Res Protoc. 1998; 3: 183-91.
 
Willker W, Flögel U, Leibfritz D.
A 1H/13C inverse 2D method for the analysis of the polyamines putrescine, spermidine and spermine in cell extracts and biofluids.
NMR Biomed. 1998; 11: 47-54.
 
Pfeuffer J, Flögel U, Dreher W, Leibfritz D.
Restricted diffusion and exchange of intracellular water: theoretical modelling and diffusion time dependence of 1H NMR measurements on perfused glial cells.
NMR Biomed. 1998; 11: 19-31.
 
Pfeuffer J, Flögel U, Leibfritz D.
Monitoring of cell volume and water exchange time in perfused cells by diffusion-weighted 1H NMR spectroscopy.
NMR Biomed. 1998; 11: 11-8.
 
Serkova N, Christians U, Flögel U, Pfeuffer J, Leibfritz D.
Assessment of the mechanism of astrocyte swelling induced by the macrolide immunosuppressant sirolimus using multinuclear nuclear magnetic resonance spectroscopy.
Chem Res Toxicol. 1997; 10: 1359-63.
 
Flögel U, Willker W, Leibfritz D.
Determination of de novo synthesized amino acids in cellular proteins revisited by 13C NMR spectroscopy.
NMR Biomed. 1997; 10: 50-8.
 
Benters J, Flögel U, Schäfer T, Leibfritz D, Hechtenberg S, Beyersmann D.
Study of the interactions of cadmium and zinc ions with cellular calcium homoeostasis using 19F NMR spectroscopy.
Biochem J. 1997; 322: 793-9.
 
Willker W, Flögel U, Leibfritz D.
Ultra-high-resolved HSQC spectra of multiple 13C-labeled biofluids.
J Magn Reson. 1997; 125: 216-9.
 
Alves PM, Flögel U, Brand A, Leibfritz D, Carrondo MJ, Santos H, Sonnewald U.
Immobilization of primary astrocytes and neurons for online monitoring of biochemical processes by NMR.
Dev Neurosci. 1996; 18: 478-83.
 
Flögel U, Willker W, Engelmann J, Niendorf T, Leibfritz D.
Adaptation of cellular metabolism to anisosmotic conditions in a glial cell line, as assessed by 13C NMR spectroscopy.
Dev Neurosci. 1996; 18: 449-59.
 
Flögel U, Niendorf T, Serkowa N, Brand A, Henke J, Leibfritz D.
Changes in organic solutes, volume, energy state, and metabolism associated with osmotic stress in a glial cell line: a multinuclear NMR study.
Neurochem Res. 1995; 20: 793-802.
 
Flögel U, Willker W, Leibfritz D.
Regulation of intracellular pH in neuronal and glial tumour cells, studied by multinuclear NMR spectroscopy.
NMR Biomed. 1994; 7: 157-66.
 
Flögel U, Leibfritz D.
Response of 3T3 cells on stimulation of adenylate cyclase by forskolin, studied by multinuclear NMR spectroscopy.
Biol Chem Hoppe Seyler. 1993; 374: 211-6.
 
 
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